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THE ASSAY OF ATP:UREA AMIDOLYASE IN WHOLE CELLS OF CHLORELLA
Article first published online: 2 MAY 2006
DOI: 10.1111/j.1469-8137.1976.tb01476.x
1469-8137/asset/cover.gif?v=1&s=570a3a280b205b8cd6b43e7a8ee052e7536c7006 "New Phytologist")
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How to Cite
THOMAS, E. M. and SYRETT, P. J. (1976), THE ASSAY OF ATP:UREA AMIDOLYASE IN WHOLE CELLS OF CHLORELLA. New Phytologist, 76: 409–413. doi: 10.1111/j.1469-8137.1976.tb01476.x
Publication History
- Issue published online: 2 MAY 2006
- Article first published online: 2 MAY 2006
- Received 22 October 1975
- Abstract
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SUMMARY
In some strains of Chlorella (Ie, 8k, 9a, 21), urea amidolyase activity can be assayed by incubating frozen/thawed cells with 14C-urea and ATP. In other strains (8b, 8c, 8p, nm, nn), ATP is ineffective in the assay with frozen/thawed cells but phosphoenolpyruvate is effective. Avidin, which inhibits urea amidolyase activity by complexing with biotin, inhibits the activity in cells of the first group of strains but not in cells of the second group. These differences in behaviour are attributed to differences in the permeability of cell walls; they correlate with other differences described earlier.