SEED PROTEIN HOMOLOGY AND ELUCIDATION OF SPECIES RELATIONSHIPS IN PHASEOLUS AND VIGNA SPECIES

Authors

  • SUMAN SAHAI,

    1. Division of Genetics, Indian Agricultural Research Institute, New Delhi-110012, India.
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      Present address and request for reprints: Dr Suman Sahai, Department of Biochemistry, All India Institute of medical Sciences, New Delhi-110016, India.

  • R. S. RANA

    1. Division of Genetics, Indian Agricultural Research Institute, New Delhi-110012, India.
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    • Dr R. S. Rana, Head Dept. of Genetics & Plant Physiology, CSSRI, Karnal, Harayana, India.


SUMMARY

Electrophoresis of seed proteins from Phaseolus and Vigna species was carried out on poly-acrylamide gels with cationic and anionic systems. The species were grouped into seven clusters. On the basis of matrices of similarity index, keeping sixty as the minimum intra-cluster similarity coefficient, a three-dimensional model was constructed to represent the relationships amongst the clusters. Five Asiatic species including Phaseolus aureus, P. mungo, P. sublobatus, P. angularis and P. calcaratus formed a cluster which was separate from a cluster of American species. The Vigna species forming a third cluster showed greater affinity with the Asiatic Phaseolus species than with the American ones. P. aconitifolius, a form cultivated in India, and two American species, P. lunatus and P. lathyroides, were distinct between themselves and also from other species.

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