Trifolium repens (white clover) or Allium cepa (onion) infected with the mycorrhizal fungus Glomus mosseae were grown in split-plate cultures, so that nutrients could be supplied to the external hyphae only. The uptake and translocation of 32P, 65Zn and 35S by hyphae of the fungus was measured.

A lag phase of 2-3 days in the appearance of 32P in the shoot was caused by a delay in the uptake and translocation of P by the hyphae, rather than in transfer from fungus to host.

The calculated maximum translocation rate of P for onion was 6.4 × 10−16 mole s−1, and the P flux in the hyphae about 2 × 10−10 mole cm−2 s−1. The molar amounts of P, S and Zn translocated were in the ratio of 35:5:1 and the mean fluxes in the ratio of 50:8:1 with clover.