THE EFFECT OF COPPER ON NITROGEN FIXATION IN SUBTERRANEAN CLOVER (TRIFOLIUM SUBTERRANEUM)

Authors


SUMMARY

Copper deficiency decreased nitrogen fixation in subterranean clover. Three lines of evidence support this conclusion. Firstly, while both copper and nitrogen application increased the growth of the legume the interaction between copper and nitrogen on growth was negative. Secondly, the application of copper increased the concentrations of both total and protein nitrogen in the plant. Lastly, nitrogen fixation as measured by the acetylene reduction assay increased with copper application to the soil.

Ancillary