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THE ACID INVERTASES OF THE DEVELOPING THIRD LEAF OF OAT
II. CHANGES IN THE ACTIVITIES OF SOLUBLE ISOENZYMES
Article first published online: 2 MAY 2006
Volume 88, Issue 2, pages 279–288, June 1981
How to Cite
GREENLAND, A. J. and LEWIS, D. H. (1981), THE ACID INVERTASES OF THE DEVELOPING THIRD LEAF OF OAT. New Phytologist, 88: 279–288. doi: 10.1111/j.1469-8137.1981.tb01724.x
- Issue published online: 2 MAY 2006
- Article first published online: 2 MAY 2006
- (Accepted 29 September 1980)
Soluble acid invertases, obtained from the developing third leaf of oat, were fractionated by gel filtration and ion-exchange chromatography. Gel filtration separated two peaks. Isoenzyme II (85 × 103 daltons) was the dominant form in the non-emerged leaf but decreased in activity after leaf emergence. The activity of isoenzyme I (143 × 103 daltons) remained constant throughout leaf development and was dominant in mature tissues. Ion-exchange chromatography separated two discrete peaks (A and B) and a composite peak (C), comprised several isoenzymes. The activities associated with A, B and C varied considerably during leaf development. One or more of the isoenzymes of invertase in peak C was the predominant invertase at all stages of leaf development. These observations are discussed in relation to the role and control of synthesis of invertases during development.