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SUMMARY

Rapid separation and determination of tryptophan, N-formylkynurenine, kynurenine, 3-hydroxykynurenine, 3-hydroxyanthranilic acid, nicotinic acid, nicotinamide, trigonelline, kynurenic acid and xanthurenic acid from pea seedling using reverse-phase high-pressure liquid chromatography is described. Accuracy of the determinations was better than 5 % and less than 50 pmol of each metabolite could be measured reliably.