Several criteria have been established for more effective utilization of inoculum of Pisolithus tinctorius (Mich. exPers.) Ckr. & Couch and of Paxillus involutus (Batsch. ex Fr.) Fr. P. tinctorius inoculum prepared on vermiculite-peat moss medium can be stored up to 19 weeks at 3 °C without damage but P. involutus inoculum cannot be stored for more than two weeks. At 24 °C neither inoculum retained viability for long, emphasizing the need for such inoculum to be applied to host roots in a way which permits rapid infection.
A blended inoculum prepared from P. tinctorius grown in liquid culture had a maximum activity, at the age of three weeks, which coincided with its maximum ATP concentration, suggesting that ATP might prove to be a valuable guide to inoculum potential. This inoculum is most tolerant of blending procedures and establishes itself better on both nutrient-rich and nutrient-poor substrates when previously grown on nutrient-poor media.
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