DEVELOPMENT OF ‘EPIDERMAL’ GRAFTS ON CULTURED INTERNODES OF DATURA STRAMONIUM

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SUMMARY

This paper describes a novel system of graft assembly in vitro performed on excised Datura stramonium internodes. The grafted material is a 2 × 10 mm strip of superficial tissue consisting of the epidermis plus three to seven layers of underlying tissue and the technique is therefore called ‘epidermal’ grafting. The excision of the strip of superficial tissue initiates cell division in the wounded tissue. At 10 d, control internodes, where tissue was excised but not replaced, had significantly higher cell numbers than the corresponding homografts. The assembly of the graft therefore appears to limit the cell division caused by tissue injury. Only two cell types are brought into contact during ‘epidermal’ graft assembly, thereby circumventing the problems associated with the study of the union of multiple cell types. This technique, therefore, represents a simplified system in which the cellular interactions during graft formation can be studied.

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