This work was funded by the Australian Meat Research Committee. We thank R. Butler, G. J. Butler, S. Eustace, J. H. Warcup and M. Clements for gifts of orchid seed and R. Butler for assisting in the collection of orchid plants. We are also grateful to M. Clements and Dr J. H. Warcup for valuable discussion and to Dr M. L. Reed for reading the manuscript.
The mycorrhizal relationship of multinucleate rhizoctonias from non-orchids with Microtis (Orchidaceae)
Article first published online: 28 APR 2006
Volume 108, Issue 2, pages 205–209, February 1988
How to Cite
Milligan, M. J. and Williams, P. G. (1988), The mycorrhizal relationship of multinucleate rhizoctonias from non-orchids with Microtis (Orchidaceae). New Phytologist, 108: 205–209. doi: 10.1111/j.1469-8137.1988.tb03697.x
Present address: Biological and Chemical Research Institute, Rydalmere 2116, N.S.W., Australia.
- Issue published online: 28 APR 2006
- Article first published online: 28 APR 2006
- (Received 13 April 1987; accepted 2 September 1987)
- Key words: Orchid mycorrhiza, Mirotis, Sebacina vermifera, symbiotic germination tests
Multinucleate rhizoctonias isolated from the roots of non-orchid plants were used in symbiotic germination tests with seed of 18 orchid species from 10 genera. The rhizoctonias only stimulated germination of Microtis. Investigation of the natural mycosymbionts of adult Microtis failed to yield fungi similar to the original multinucleate rhizoctonias: 27 Microtis plants collected at seven sites in the Sydney region yielded 26 binucleate rhizoctonias tentatively identified as Tulasnella calospora (Boudier) Juel and one very slow growing multinucleate rhizoctonia tentatively identified as Sebacina vermifera Oberwinkler. Fungi closely resembling the original multinucleate rhizoctonias were isolated by baiting the soil with subterranean clover (Trifolium subterraneum L.) plants at three of the seven Microtis sites.