Max-Planck-Institut für Züchtungsforschung, Abt. Biochemie, D-5000 köln 30, Federal Republic of Germany.
Carbohydrates on the surface of urediniospore- and basidiospore-derived infection structures of heteroecious and autoecious rust fungi
Article first published online: 28 APR 2006
Volume 119, Issue 4, pages 527–534, December 1991
How to Cite
FREYTAG, S. and MENDGEN, K. (1991), Carbohydrates on the surface of urediniospore- and basidiospore-derived infection structures of heteroecious and autoecious rust fungi. New Phytologist, 119: 527–534. doi: 10.1111/j.1469-8137.1991.tb01044.x
- Issue published online: 28 APR 2006
- Article first published online: 28 APR 2006
- (Received 24 April 1991; accepted 21 August 1991)
- Rust fungi;
- infection structures;
- surface carbohydrates;
Nine flucirescein isothiocyanate-labelled lectins with affinity towards different carbohydrates were used to probe the surface carbohydrates of infection structures in vitro, derived from urediniospores and basidiospores of an autoecious rust species, Uromyces viciae-fabae (Pers. J Schroet., and of a heteroecious rust species, Uromyces rumicis (Schum.) Wint. Lectin binding was quantified by measuring fluorescence photometrically.
All lectins bound in a characteristic pattern to the infection structures of the respective spore types of each rust fungus. Differences were especially obvious between those infection structures normally produced inside leaf tissue, namely the urediniospore-derived substomatal vesicles with infection hyphae, and the basidiospore-derived intraepidermal vesicles. The dikaryotic stage and the monokaryotic stage of the heteroecious fungus differed mainly in their affinity for the leotins from Bandeira simplicifolin and Lotus tetragonulobus.
A statistical analysis comparing the binding of the lectins to infection structures of Two rust fungi suggested that cell surface composition is determined by nuclear condition. The monokaryotic stages of both rust fungi have a higher degree of similarity than the dikaryotic and monokaryotic stages of the same rust fungus.