The effect of infection by Erysiphe pisi DC on acid and alkaline invertase activities and aspects of starch biochemistry in leaves of Pisum sativum L.

Authors

  • TREVOR STORR,

    1. Department of Biology, Biomedical Sciences Building, The University of Southampton, Southampton, SO9 3TU, U.K.
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  • J. L. HALL

    Corresponding author
    1. Department of Biology, Biomedical Sciences Building, The University of Southampton, Southampton, SO9 3TU, U.K.
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To whom correspondance and reprint requests should be addressed

summary

The acid and alkaline invertases of mature leaves of Pisum sativum were partially purified and characterized prior to determining the effects of powdery mildew (Erysiphe pisi) infection on their activities. Seven days after inoculation, infection resulted in significant increases in the activities of both acid and alkaline invertases in whole leaves of cv. Onward, but a significant increase in the activity of only alkaline invertase in isolated mesophyll of cv. Argenteum. Activity of α-glucosidase was determined in leaves and it was concluded that this enzyme could not be responsible for the increased sucrose hydrolase activity observed in host tissues. Seven days after inoculation, infection caused a significant decrease in leaf starch content, a decrease in the activity of ADP-glucose pyrophosphorylase, and no significant change in the activity of α-glucan phosphorylase or content of inorganic phosphate. It was proposed that the decreased content of starch is a result of decreased starch synthesis.

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