Isolation and purification of intralamellar vesicles from Lemna minor L. chloroplasts

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SUMMARY

Intralamellar vesicles (IV) from chloroplasts of Lemna minor L. have been isolated and purified on a sucrose gradient, following sonication. Electron microscopy studies and lipid composition analyses suggest that IV are different in structure and in lipid composition from plastoglobuli. The presence of a high galactolipid composition (70%) suggests that IV are surrounded by a membrane (unit or half-unit). The ratio of diacylgalactosylglycerol/diacyldigalactosylglycerol (3.9) of IV is similar to that of thylakoid membranes facing the stroma.

Ancillary