Role of superoxide, lipid peroxidation and superoxide dismutase in membrane perturbation during loss of viability in seeds of Shorea robusta Gaertn.f.

Authors

  • K. S. KRISHNA CHAITANYA,

    1. School of Studies in Life Sciences, Pt. Ravishankar Shukla University, Raipur - 492 010 (M.P.), India
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  • SUBHASH CHANDRA NAITHANI

    Corresponding author
    1. School of Studies in Life Sciences, Pt. Ravishankar Shukla University, Raipur - 492 010 (M.P.), India
      *To whom correspondence should be addressed.
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*To whom correspondence should be addressed.

SUMMARY

Recalcitrant seeds of Shorea robusta (sal) exhibit 100% viability up to 4 d after maturity. The rapid loss of viability after 4 d is associated with the reduction in moisture content below the lowest safe moisture content (37°o). Seed becomes non-viable on 8 d. Increased leakage of electrolytes in seeds and lipid peroxidation in embryonic axes was discernible immediately from 0 d. In embryonic axes, very low levels of superoxide (O2) were maintained up to 4 d and a sharp increase was registered up to 7 d. It is suggested that loss of moisture content in sal seeds below 37% (after 4 d) induces substantial leakage loss probably due to increased lipid peroxidation and O2) radical formation which are responsible for severe membrane perturbations leading to rapid loss of viability. In embryonic axes SOD activity was recorded only in 100% viable seeds.

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