Nitrate depletion and pH changes induced by the extraradical mycelium of the arbuscular mycorrhizal fungus Glomus intraradices grown in monoxenic culture

Authors

  • B. BAGO,

    Corresponding author
    1. Centre de Recherche en Biologie Forestière, Faculté de Foresterie et de Géomatique, Pavilion C.-E. Marchand, Université Laval, Québec, G1K 7P4 Canada
      *To whom correspondence should be addressed.
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  • H. VIERHEILIG,

    1. Centre de Recherche en Biologie Forestière, Faculté de Foresterie et de Géomatique, Pavilion C.-E. Marchand, Université Laval, Québec, G1K 7P4 Canada
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  • Y. PICHÉ,

    1. Centre de Recherche en Biologie Forestière, Faculté de Foresterie et de Géomatique, Pavilion C.-E. Marchand, Université Laval, Québec, G1K 7P4 Canada
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  • C. AZCÓN-AGUILAR

    1. Departamento de Microbiologia del Suelo y Sistemas Simbióticos Estación Experimental del Zaidin (CSIC), Profesor Albareda 1, 18008 Granada, Spain
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*To whom correspondence should be addressed.

summary

The effect of the extraradical mycelium of the arbuscular mycorrhizal (AM) fungus Glomus intraradices Smith & Schenck on nitrate uptake and on the pH of the medium was studied in a monoxenic culture with tomato (Lycopersicon esculentum Mill. var. Vendor) roots obtained from root organ culture. The symbiosis was established in compartmented Petri dishes containing agar media amended with the pH indicator bromocresol purple. A pattern of pH changes was revealed as the symbiosis progressed in the media of the Petri dish compartments containing the dual, arbuscular-mycorrhizal fungi/root, culture as well as in the media of the hyphae, root-free compartments, in which the extraradical hyphae developed extensively, coming from the compartment containing the symbiosis. The colour changes in the media were measured spectrophotometrically, whilst maintaining the monoxenic conditions. The extraradical hyphae of G. intraradices strongly increased the pH of nutrient-free medium when supplied with nitrate, whereas the pH decreased m the absence of this N source. The hyphae developing from germinated spores and growing in axenic, nitrate-amended media did not induce any increase in pH. Nitrogen analysis revealed that a depletion of nitrate in the media accompanied increased pH.

These results point towards an active uptake of nitrate by the extraradical mycelium of G. intraradices, probably coupled to a H+-symport mechanism. The pH changes induced by AM fungal hyphae and the possible influence of the establishment of a functional symbiosis on these pH changes are discussed.

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