The poplar cultivar ‘Peace’ (Populus trichocarpa×koreana) displays abnormal stomatal behaviour with a lack of sensitivity to exogenous abscisic acid (ABA) in mature leaves. We report details of the leaf-age dependency of this feature, and present the responses of stomata to diverse closing stimuli in addition to ABA. Soil water depletion induced complete stomatal closure of the youngest leaves, whereas the oldest exhibited almost no closure. By contrast, no such age effect was observed in stomatal sensitivity to ABA: a complete lack of closure was observed on all leaves, even with 10-3 M ABA. Moreover, a preconditioning by drought did not restore the ability of stomata to close in response to ABA. Supplying detached leaves with Ca2+ (5 × 10-2 M) was effective in inducing stomatal closure in the youngest leaves, and reproduced the age-dependent drought response. These results support the hypothesis that drought control of stomatal conductance in ‘Peace’ is ABA-independent, and could involve calcium ions. A loss of stomatal sensitivity to calcium, with leaf maturation, could explain the dependency of stomatal closure on leafage. Xylem sap concentrations of calcium were lower than those required to close stomata, and no drought-induced increase was recorded. The mature leaves were also insensitive to increased ambient CO2, but darkness promoted partial stomatal closure.