The Rg-1 encoded regeneration capacity of tomato is not related to an altered cytokinin homeostasis
Author for correspondence: Els Prinsen Tel: +32 3 8202266 Fax: +32 3 8202271 Email: email@example.com
- • Cytokinin (CK) metabolism was analyzed in tomato (Lycopersicon esculentum) Rg-1 hybrids during in vitro shoot organogenesis from root explants.
- • Data were obtained by combining physicochemical analysis with quantification and in situ detection methods.
- • Although exogenous zeatin is added in all classical regeneration protocols, we show here that regenerating (Rg+) tomato explants did not require an exogenous CK source for regeneration. Irrespective of the presence or absence of exogenous zeatin, the endogenous CK levels were not affected by Rg-1 in the initial explants or in the early callus phase. In a later stage, and related to the presence of numerous shoots, the Rg+ explants showed much lower endogenous CK concentrations than the nonregenerating (rg−) explants. Cells of rg− explants were not able to differentiate, despite their high endogenous CK content, and did not respond to exogenously applied CKs.
- • We show that the insensitivity of rg− explants to a hormonal signal, normally initiating regeneration, is not related to an altered endogenous CK metabolism. We therefore postulate that Rg-1 action involves a regeneration-specific CK receptor or a regeneration-specific CK signal transduction pathway.