24-Methyl/methylene sterols increase in monoxenic roots after colonization by arbuscular mycorrhizal fungi
Article first published online: 22 APR 2004
Volume 163, Issue 1, pages 159–167, July 2004
How to Cite
Fontaine, J., Grandmougin-Ferjani, A., Glorian, V. and Durand, R. (2004), 24-Methyl/methylene sterols increase in monoxenic roots after colonization by arbuscular mycorrhizal fungi. New Phytologist, 163: 159–167. doi: 10.1111/j.1469-8137.2004.01075.x
- Issue published online: 22 APR 2004
- Article first published online: 22 APR 2004
- Received: 5 December 2003 Accepted: 19 February 2004; doi: 10.1111/j.1469-8137.2004.01075.x
- Daucus carota (carrot);
- Cichorium intybus (chicory);
- monoxenic cultures;
- Ri T-DNA-transformed roots
- • Characteristic sterols of transformed carrot (Daucus carota) and chicory (Cichorium intybus) roots colonized by different strains of arbuscular mycorrhizal (AM) fungi were identified.
- • Sterols were extracted, analysed and identified by gas chromatography/mass spectrometry (GC-MS) from monoxenic cultures of mycorrhizal and nonmycorrhizal roots. After colonization by Glomus intraradices, Glomus proliferum and Glomus sp., carrot and chicory roots exhibited a significantly higher 24-methyl/methylene sterol content. A correlation was established between the content of the sum of 24-methyl cholesterol, 24-methylene cholesterol and 24-methyl desmosterol.
- • This study clearly established that the increment of these characteristic sterols is an appropriate indicator of colonization by AM fungi of transformed roots.
- • Metabolic origin and specificity of these sterols in mycorrhizal roots was researched. The 24-methyl/methylene sterol increase was observed only when the interaction between fungus and plant was completely established and the fungus was present inside the roots.