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Keywords:

  • sterol;
  • Glomus;
  • Daucus carota (carrot);
  • Cichorium intybus (chicory);
  • monoxenic cultures;
  • Ri T-DNA-transformed roots

Summary

  • • 
    Characteristic sterols of transformed carrot (Daucus carota) and chicory (Cichorium intybus) roots colonized by different strains of arbuscular mycorrhizal (AM) fungi were identified.
  • • 
    Sterols were extracted, analysed and identified by gas chromatography/mass spectrometry (GC-MS) from monoxenic cultures of mycorrhizal and nonmycorrhizal roots. After colonization by Glomus intraradices, Glomus proliferum and Glomus sp., carrot and chicory roots exhibited a significantly higher 24-methyl/methylene sterol content. A correlation was established between the content of the sum of 24-methyl cholesterol, 24-methylene cholesterol and 24-methyl desmosterol.
  • • 
    This study clearly established that the increment of these characteristic sterols is an appropriate indicator of colonization by AM fungi of transformed roots.
  • • 
    Metabolic origin and specificity of these sterols in mycorrhizal roots was researched. The 24-methyl/methylene sterol increase was observed only when the interaction between fungus and plant was completely established and the fungus was present inside the roots.