Over-expression of phenol-oxidising peroxidases alters the UV-susceptibility of transgenic Nicotiana tabacum
Author for correspondence: Marcel A. K. Jansen Tel: +353 (0)21 4904558 Fax: +353 (0)21 4251256 Email: M.Jansen@UCC.IE
- • Class III peroxidases catalyse the oxidative crosslinking of UV-absorbing phenolics. The effect of changes in the activity of phenol oxidising peroxidases (EC 22.214.171.124) on UV-tolerance in Nicotiana tabacum plants has been determined.
- • The UV-sensitivity of transgenic N. tabacum lines, altered in their peroxidase expression pattern, was studied by measuring radiation effects on photosynthetic efficiency.
- • Analysis of the effect of UV-radiation on the relative variable chlorophyll fluorescence showed that the SPI-2 line, which over-expresses a defence-related cationic peroxidase, is markedly UV-tolerant. By contrast, the ROPN3-line, which overexpresses a synthetic horseradish peroxidase-C gene, was found to be UV-sensitive. The increased activity of indole-3-acetic acid (IAA) inducible peroxidases in homozygous IAA-overproducing transgenic plants was also found to correlate with UV-sensitivity.
- • It is concluded that only specific peroxidase isozymes, through their effects on phenolic metabolism, contribute to the UV protection response. Thus, the analysis of the role of isozymes in UV-protection addresses fundamental questions of isozyme diversity and/or redundancy in relation to phenolic substrates.