Poplar genes encoding fasciclin-like arabinogalactan proteins are highly expressed in tension wood
Article first published online: 1 SEP 2004
Volume 164, Issue 1, pages 107–121, October 2004
How to Cite
Lafarguette, F., Leplé, J.-C., Déjardin, A., Laurans, F., Costa, G., Lesage-Descauses, M.-C. and Pilate, G. (2004), Poplar genes encoding fasciclin-like arabinogalactan proteins are highly expressed in tension wood. New Phytologist, 164: 107–121. doi: 10.1111/j.1469-8137.2004.01175.x
- Issue published online: 1 SEP 2004
- Article first published online: 1 SEP 2004
- Received: 29 April 2004 Accepted: 24 June 2004
- arabinogalactan protein;
- fasciclin-like AGP;
- tension wood;
- xylem differentiation
- • Fifteen poplar cDNA encoding fasciclin-like arabinogalactan proteins (PopFLAs) were finely characterized, whereas the presence of arabinogalactan proteins (AGPs) was globally assessed during wood formation.
- • PopFLAs transcript accumulation was analysed through EST distribution in cDNA libraries, semi-quantitative RT-PCR, microarray experiment and Northern blot analysis. Similarly, AGPs contents were globally quantified by rocket electrophoresis. AGPs accumulation was further examined by Western blotting and immunocytolocalization.
- • Ten PopFLAs were specifically expressed in tension wood (TW) and not expressed in the cambial zone. Rocket electrophoresis revealed important AGPs accumulation in TW xylem. An anti-AGPs specific antibody recognized two proteins preferentially present in the cell wall-bound fraction from TW. Immunocytochemistry revealed a strong labelling close to the inner part of the G-layer of TW fibres.
- • PopFLAs are expressed in xylem and many are up-regulated in TW. It is suggested that some PopFLAs accumulating at the inner side of the G-layer may have a specific function in the building of this layer. PopFLAs expression may therefore be linked to the specific mechanical properties of TW.