Poplar genes encoding fasciclin-like arabinogalactan proteins are highly expressed in tension wood

Authors

  • Florian Lafarguette,

    1. Equipe ‘Formation des Parois Lignifiées’, Unité Amélioration, Génétique et Physiologie Forestières, INRA Orléans, Avenue de la Pomme de Pin, BP 20 619 Ardon, F-45 166 Olivet Cedex, France;
    2. Present address: Forest Biology Research Center, Université Laval, Québec, QC, Canada, G1K 7P4;
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  • Jean-Charles Leplé,

    Corresponding author
    1. Equipe ‘Formation des Parois Lignifiées’, Unité Amélioration, Génétique et Physiologie Forestières, INRA Orléans, Avenue de la Pomme de Pin, BP 20 619 Ardon, F-45 166 Olivet Cedex, France;
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  • Annabelle Déjardin,

    1. Equipe ‘Formation des Parois Lignifiées’, Unité Amélioration, Génétique et Physiologie Forestières, INRA Orléans, Avenue de la Pomme de Pin, BP 20 619 Ardon, F-45 166 Olivet Cedex, France;
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  • Françoise Laurans,

    1. Equipe ‘Formation des Parois Lignifiées’, Unité Amélioration, Génétique et Physiologie Forestières, INRA Orléans, Avenue de la Pomme de Pin, BP 20 619 Ardon, F-45 166 Olivet Cedex, France;
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  • Guy Costa,

    1. Laboratoire de Chimie des Substances Naturelles, Groupe de Glycobiologie Forestière, 123, Avenue Albert Thomas, F-87 060 Limoges Cedex, France
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  • Marie-Claude Lesage-Descauses,

    1. Equipe ‘Formation des Parois Lignifiées’, Unité Amélioration, Génétique et Physiologie Forestières, INRA Orléans, Avenue de la Pomme de Pin, BP 20 619 Ardon, F-45 166 Olivet Cedex, France;
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  • Gilles Pilate

    1. Equipe ‘Formation des Parois Lignifiées’, Unité Amélioration, Génétique et Physiologie Forestières, INRA Orléans, Avenue de la Pomme de Pin, BP 20 619 Ardon, F-45 166 Olivet Cedex, France;
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Author for correspondence: Jean-Charles Leplé Tel: +33 2 38 41 78 37 Fax: +33 2 38 41 78 79 Email: Jean-Charles.Leple@orleans.inra.fr

Summary

  • • Fifteen poplar cDNA encoding fasciclin-like arabinogalactan proteins (PopFLAs) were finely characterized, whereas the presence of arabinogalactan proteins (AGPs) was globally assessed during wood formation.
  • • PopFLAs transcript accumulation was analysed through EST distribution in cDNA libraries, semi-quantitative RT-PCR, microarray experiment and Northern blot analysis. Similarly, AGPs contents were globally quantified by rocket electrophoresis. AGPs accumulation was further examined by Western blotting and immunocytolocalization.
  • • Ten PopFLAs were specifically expressed in tension wood (TW) and not expressed in the cambial zone. Rocket electrophoresis revealed important AGPs accumulation in TW xylem. An anti-AGPs specific antibody recognized two proteins preferentially present in the cell wall-bound fraction from TW. Immunocytochemistry revealed a strong labelling close to the inner part of the G-layer of TW fibres.
  • • PopFLAs are expressed in xylem and many are up-regulated in TW. It is suggested that some PopFLAs accumulating at the inner side of the G-layer may have a specific function in the building of this layer. PopFLAs expression may therefore be linked to the specific mechanical properties of TW.

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