The use of conventional and quantitative real-time PCR assays for Polymyxa graminis to examine host plant resistance, inoculum levels and intraspecific variation
Article first published online: 20 DEC 2004
Volume 165, Issue 3, pages 875–885, March 2005
How to Cite
Ward, E., Kanyuka, K., Motteram, J., Kornyukhin, D. and Adams, M. J. (2005), The use of conventional and quantitative real-time PCR assays for Polymyxa graminis to examine host plant resistance, inoculum levels and intraspecific variation. New Phytologist, 165: 875–885. doi: 10.1111/j.1469-8137.2004.01291.x
- Issue published online: 20 DEC 2004
- Article first published online: 20 DEC 2004
- Received: 30 July 2004 Accepted: 4 October 2004
- plant resistance;
- Polymyxa graminis;
- real-time PCR;
- Soil-borne cereal mosaic virus (SBCMV);
- Triticum monococcum
- • A real-time PCR protocol based on 18S rDNA sequences was developed to provide a specific, sensitive and quantitative assay for the root-infecting virus vector Polymyxa graminis.
- • The assay was calibrated with zoospore suspensions and inoculated roots and then shown to work with naturally infected plant roots and infested soil. Both the temperate P. graminis ribotypes previously described are detected but are not distinguished. DNA from related plasmodiophorids and from a range of fungi and plants was not detected.
- • Different genotypes of Triticum were grown in a soil infested with P. graminis and Soil-borne cereal mosaic virus (SBCMV). The genotypes differed in susceptibility to P. graminis, the least susceptible being the Triticum monococcum accession K-58505.
- • Conventional PCR assays and sequencing of amplified rDNA fragments showed that P. graminis isolates infecting wheat were mostly, but not exclusively, of ribotype II. Ribotype II was clearly associated with SBCMV transmission and seems to occur preferentially on wheat whereas ribotype I is mostly associated with barley.