Seasonal adaptations of the tuberous roots of Ranunculus asiaticus to desiccation and resurrection by changes in cell structure and protein content
Article first published online: 12 JAN 2005
Volume 166, Issue 1, pages 193–204, April 2005
How to Cite
Kamenetsky, R., Peterson, R. L., Melville, L. H., Machado, C. F. and Bewley, J. D. (2005), Seasonal adaptations of the tuberous roots of Ranunculus asiaticus to desiccation and resurrection by changes in cell structure and protein content. New Phytologist, 166: 193–204. doi: 10.1111/j.1469-8137.2004.01306.x
- Issue published online: 12 JAN 2005
- Article first published online: 12 JAN 2005
- Received: 13 August 2004 Accepted: 1 November 2004
- cell walls;
- storage proteins
- • The annual developmental cycle of tuberous roots of Ranunculus asiaticus was studied with respect to structure and content of their cells, to understand how these roots are adapted to desiccation, high temperature and rehydration.
- • Light microscopy, histochemical analysis, and protein analyses by SDS-PAGE were employed at eight stages of annual root development.
- • During growth and maturation of the roots, cortical cells increased in size and their cell walls accumulated pectin materials in a distinct layer to the inside of the primary walls, with pits between adjoining cells. The number of starch granules and protein bodies also increased within the cells. Several discrete proteins accumulated. Following quiescence and rehydration of the roots there was a loss of starch and proteins from the cells, and cell walls decreased in thickness.
- • The resurrection geophyte R. asiaticus possesses desiccation-tolerant annual roots. They store carbon and nitrogen reserves within their cells, and pectin within the walls to support growth of the plant following summer quiescence and rehydration.