Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures

Authors

  • Annalisa Tassoni,

    1. Department of Biology e.s and Interdepartmental Centre for Biotechnology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy;
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  • Silvia Fornalè,

    1. Department of Biology e.s and Interdepartmental Centre for Biotechnology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy;
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  • Marina Franceschetti,

    1. Department of Biology e.s and Interdepartmental Centre for Biotechnology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy;
    2. Institute of Food Research, Norwich Research Park, Colney Lane, Norwich, NR4 7UA, UK
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  • Federica Musiani,

    1. Department of Biology e.s and Interdepartmental Centre for Biotechnology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy;
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  • Anthony J. Michael,

    1. Institute of Food Research, Norwich Research Park, Colney Lane, Norwich, NR4 7UA, UK
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  • Barry Perry,

    1. Institute of Food Research, Norwich Research Park, Colney Lane, Norwich, NR4 7UA, UK
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  • Nello Bagni

    Corresponding author
    1. Department of Biology e.s and Interdepartmental Centre for Biotechnology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy;
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Author for correspondence: Nello Bagni Tel: +39 0512091280 Fax: +39 051242576 Email: bagninel@alma.unibo.it

Summary

  • • Here the effect of jasmonic acid, methyljasmonate and Na-orthovanadate on the production of resveratrol was studied in Vitis vinifera cv. Barbera cell suspension cultures.
  • • Na-orthovanadate at 0.1 mm and 1 mm concentration was efficient in promoting the production and/or accumulation and release in the culture medium of cis-resveratrol while trans-resveratrol levels were not affected by this treatment.
  • • Methyljasmonate was highly effective in stimulating both trans- and cis-resveratrol endogenous accumulation, as well as their release into the culture medium. Cis-resveratrol was absent or detected in very low amounts in the controls. Jasmonic acid was less efficient than methyljasmonate in promoting endogenous resveratrol accumulation, but it stimulated the release in the culture medium especially of cis-resveratrol.
  • • Gel analysis was performed on control and 10 µm MeJA treated cell suspensions. Results showed an up-regulation of the stilbene synthase demonstrating that MeJA stimulated the synthesis ex-novo of this protein.

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