Temperature constraints on the growth and functioning of root organ cultures with arbuscular mycorrhizal fungi

Authors

  • Mayra E. Gavito,

    Corresponding author
    1. Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, C/Profesor Albareda 1, E-18008 Granada, Spain;
    2. Present address: Centro de Investigaciones en Ecosistemas, Universidad Nacional Autónoma de México, Apartado Postal 27–3 C. P. 58090, Morelia, Michoacán, Mexico
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  • Pål A. Olsson,

    1. Department of Microbial Ecology, Ecology Building, Lund University, S-223 62, Lund, Sweden;
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  • Hervé Rouhier,

    1. Department of Microbial Ecology, Ecology Building, Lund University, S-223 62, Lund, Sweden;
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  • Almudena Medina-Peñafiel,

    1. Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, C/Profesor Albareda 1, E-18008 Granada, Spain;
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  • Iver Jakobsen,

    1. Biosystems Department, Risø National Laboratory, PO Box 49, DK-4000 Roskilde, Denmark.
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  • Albert Bago,

    1. Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, C/Profesor Albareda 1, E-18008 Granada, Spain;
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  • Concepción Azcón-Aguilar

    1. Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, C/Profesor Albareda 1, E-18008 Granada, Spain;
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Author for correspondence: Mayra E. Gavito Tel: +52 56 232777 ext. 32822 Fax: +52 56 232719 E-mail: mgavito@oikos.unam.mx

Summary

  • • In this study we investigated the effects of temperature on fungal growth and tested whether the differences in fungal growth were related to the effects of temperature on carbon movement to, or within, the fungus.
  • • Growth curves and C uptake-transfer-translocation measurements were obtained for three arbuscular mycorrhizal fungi (AMF) isolates cultured within a 6–30°C temperature range. A series of experiments with a model fungal isolate, Glomus intraradices, was used to examine the effects of temperature on lipid body and 33P movement, and to investigate the role of acclimation and incubation time.
  • • Temperature effects on AMF growth were both direct and indirect because, despite clear independent root and AMF growth responses in some cases, the uptake and translocation of 13C was also affected within the temperature range tested. Root C uptake and, to a lesser extent, C translocation in the fungus, were reduced by low temperatures (< 18°C). Uptake and translocation of 33P by fungal hyphae were, by contrast, similar between 10 and 25°C.
  • • We conclude that temperature, between 6 and 18°C, reduces AMF growth, and that C movement to the fungus is involved in this response.

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