Mycorrhiza helper bacterium Streptomyces AcH 505 induces differential gene expression in the ectomycorrhizal fungus Amanita muscaria

Authors

  • Silvia D. Schrey,

    1. University of Tübingen, Faculty of Biology, Institute of Botany, Physiological Ecology of Plants, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
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  • Michael Schellhammer,

    1. University of Tübingen, Faculty of Biology, Institute of Botany, Physiological Ecology of Plants, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
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  • Margret Ecke,

    1. University of Tübingen, Faculty of Biology, Institute of Botany, Physiological Ecology of Plants, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
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  • Rüdiger Hampp,

    1. University of Tübingen, Faculty of Biology, Institute of Botany, Physiological Ecology of Plants, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
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  • Mika T. Tarkka

    Corresponding author
    1. University of Tübingen, Faculty of Biology, Institute of Botany, Physiological Ecology of Plants, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
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Author for correspondence: Mika Tarkka Tel: +40 7071 2976154 Fax: +49 7071 295635 Email: mika.tarkka@uni-tuebingen.de

Summary

  • • The interaction between the mycorrhiza helper bacteria Streptomyces nov. sp. 505 (AcH 505) and Streptomyces annulatus 1003 (AcH 1003) with fly agaric (Amanita muscaria) and spruce (Picea abies) was investigated.
  • • The effects of both bacteria on the mycelial growth of different ectomycorrhizal fungi, on ectomycorrhiza formation, and on fungal gene expression in dual culture with AcH 505 were determined.
  • • The fungus specificities of the streptomycetes were similar. Both bacterial species showed the strongest effect on the growth of mycelia at 9 wk of dual culture. The effect of AcH 505 on gene expression of A. muscaria was examined using the suppressive subtractive hybridization approach. The responsive fungal genes included those involved in signalling pathways, metabolism, cell structure, and the cell growth response.
  • • These results suggest that AcH 505 and AcH 1003 enhance mycorrhiza formation mainly as a result of promotion of fungal growth, leading to changes in fungal gene expression. Differential A. muscaria transcript accumulation in dual culture may result from a direct response to bacterial substances.

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