Characterization of a new, nonpathogenic mutant of Botrytis cinerea with impaired plant colonization capacity

Authors

  • Caroline Kunz,

    1. Laboratoire Interactions Plantes – Pathogènes (UMR 217 INRA/INA-PG/Université Pierre et Marie Curie), INA-PG, 16 rue Claude Bernard, 75231 Paris Cedex 05, France;
    Search for more papers by this author
  • Elodie Vandelle,

    1. Laboratoire de Signalisation cellulaire et moléculaire dans les réactions de défense (UMR Plante-Microbe-Environnement, INRA 1088/CNRS 5184/Université de Bourgogne), 17 rue Sully, BP 86510, 21065 Dijon cedex, France;
    Search for more papers by this author
  • Stéphane Rolland,

    1. Laboratoire de Biologie Cellulaire Fongique (UMR 5122 CNRS/INSA/Université Lyon 1), 10 rue Dubois, 69622 Villeurbanne, France;
    Search for more papers by this author
  • Benoit Poinssot,

    1. Laboratoire de Signalisation cellulaire et moléculaire dans les réactions de défense (UMR Plante-Microbe-Environnement, INRA 1088/CNRS 5184/Université de Bourgogne), 17 rue Sully, BP 86510, 21065 Dijon cedex, France;
    Search for more papers by this author
  • Christophe Bruel,

    1. Laboratoire de Biologie Cellulaire Fongique (UMR 5122 CNRS/INSA/Université Lyon 1), 10 rue Dubois, 69622 Villeurbanne, France;
    Search for more papers by this author
  • Agnès Cimerman,

    1. Laboratoire Interactions Plantes – Pathogènes (UMR 217 INRA/INA-PG/Université Pierre et Marie Curie), INA-PG, 16 rue Claude Bernard, 75231 Paris Cedex 05, France;
    Search for more papers by this author
  • Corine Zotti,

    1. Laboratoire de Biochimie et Pathologie Végétales, Université Pierre et Marie Curie (Paris VI), CC 155, 4 place Jussieu, 75252 Paris cedex 05, France
    Search for more papers by this author
  • Elisabeth Moreau,

    1. Laboratoire de Biochimie et Pathologie Végétales, Université Pierre et Marie Curie (Paris VI), CC 155, 4 place Jussieu, 75252 Paris cedex 05, France
    Search for more papers by this author
  • Régine Vedel,

    1. Laboratoire Interactions Plantes – Pathogènes (UMR 217 INRA/INA-PG/Université Pierre et Marie Curie), INA-PG, 16 rue Claude Bernard, 75231 Paris Cedex 05, France;
    Search for more papers by this author
  • Alain Pugin,

    1. Laboratoire de Signalisation cellulaire et moléculaire dans les réactions de défense (UMR Plante-Microbe-Environnement, INRA 1088/CNRS 5184/Université de Bourgogne), 17 rue Sully, BP 86510, 21065 Dijon cedex, France;
    Search for more papers by this author
  • Martine Boccara

    1. Laboratoire Interactions Plantes – Pathogènes (UMR 217 INRA/INA-PG/Université Pierre et Marie Curie), INA-PG, 16 rue Claude Bernard, 75231 Paris Cedex 05, France;
    Search for more papers by this author

Author for correspondence: Caroline Kunz Tel: +33 1 44 08 86 08 Fax: +33 1 44 08 16 31 Email: kunz@ccr.jussieu.fr

Summary

  • • Botrytis cinerea is a necrotrophic pathogen that attacks more than 200 plant species.
  • • Here, the nonpathogenic mutant A336, obtained via insertional mutagenesis, was characterized.
  • • Mutant A336 was nonpathogenic on leaves and fruits, on intact and wounded tissue, while still able to penetrate the host plant. It grew normally in vitro on rich media but its conidiation pattern was altered. The mutant did not produce oxalic acid and exhibited a modified regulation of the production of some secreted proteins (acid protease 1 and endopolygalacturonase 1). Culture filtrates of the mutant triggered an important oxidative burst in grapevine (Vitis vinifera) suspension cells, and the mutant–plant interaction resulted in the formation of hypersensitive response-like necrosis. Genetic segregation analyses revealed that the pathogenicity phenotype was linked to a single locus, but showed that the mutated gene was not tagged by the plasmid pAN7-1.
  • • Mutant A336 is the first oxalate-deficient mutant to be described in B. cinerea and it differs from all the nonpathogenic B. cinerea mutants described to date.

Ancillary