Molecular analysis of arbuscular mycorrhizal fungi colonising a semi-natural grassland along a fertilisation gradient
Article first published online: 19 JUN 2006
Volume 172, Issue 1, pages 159–168, October 2006
How to Cite
Santos, J. C., Finlay, R. D. and Tehler, A. (2006), Molecular analysis of arbuscular mycorrhizal fungi colonising a semi-natural grassland along a fertilisation gradient. New Phytologist, 172: 159–168. doi: 10.1111/j.1469-8137.2006.01799.x
- Issue published online: 19 JUN 2006
- Article first published online: 19 JUN 2006
- Received: 4 March 2006 Accepted: 2 May 2006
- arbuscular mycorrhizal fungi (AMF);
- Achillea millefolium;
- Festuca pratensis;
- small subunit (SSU) rRNA sequences;
- denaturing gradient gel electrophoresis (DGGE);
- • The community of arbuscular mycorrhizal fungi (AMF) colonizing the roots of Festuca pratensis and Achillea millefolium was characterized in a Swedish pasture at different times, along a gradient of fertilization.
- • The small subunit ribosomal RNA gene was subjected to PCR and denaturing gradient gel electrophoresis (DGGE), sequencing and phylogenetic analysis.
- • The sequences found in this study clustered in 10 discrete sequence groups, seven belonging to Glomus, two to Scutellospora and one to Diversispora.
- • A negative correlation was observed between soil mineral nitrogen and the number of AMF sequence groups in the roots. The frequency of occurrence of AMF in roots decreased dramatically between June and September. No plant-host specificity could be detected.