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Keywords:

  • Arabidopsis;
  • Bornmuellera;
  • genome size;
  • metal hyperaccumulation;
  • model system;
  • Peltaria;
  • Thlaspi;
  • transformation

Summary

  • We report on the second phase of a programme to select a relative of Arabidopsis thaliana for use in large-scale molecular genetic studies of nickel (Ni) and zinc (Zn) hyperaccumulation. We also report on the relatedness among Thlaspi caerulescens accessions and the utility of using O-acetyl-l-serine as a marker for Ni and Zn hyperaccumulation potential.
  • • 
    Twenty-seven new accessions of metal-accumulating species collected in the Czech Republic, France, Greece, Italy, Slovenia and the USA during Spring–Summer 2002 were evaluated.
  • • 
    The criteria established for selection were hyperaccumulation of metals (Ni and Zn); compact growth habit; reasonable time to flowering; production of ≥ 1000 seeds per plant; self-fertility; compact diploid genome; high sequence similarity to A. thaliana; ≥ 0.1% transformation efficiency with easy selection.
  • • 
    We conclude that the best candidate identified in the first phase was the best candidate overall: T. caerulescens accession St Félix de Pallières.