Video clip S1. A 200-nm-thick section of Synechocystis sp. 6803 wild-type cell imaged through a 120° tilt using a high-voltage transmission electron microscope (750 kV). Note the change in gold particle positions through the tilt series. Video clip S2. The Spitzenk?rper and surrounding cytoplasm of Aspergillus nidulans wild-type hypha. (a) Fifty-eight serial tomographic slices through a portion of this volume. (b) The building of a model of selected structures through the entire tomographic volume. Microvesicles (white), apical vesicles (blue), Woronin bodies (purple) and microtubules (green) are shown. (c) A rotating anaglyph movie of the model shown in (b). (d) An enlargement of the rotating anaglyph movie illustrating the spatial relationship of microtubules and apical vesicles.

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