Molecular analysis of poplar defense against herbivory: comparison of wound- and insect elicitor-induced gene expression
Article first published online: 19 SEP 2006
Volume 172, Issue 4, pages 617–635, December 2006
How to Cite
Major, I. T. and Constabel, C. P. (2006), Molecular analysis of poplar defense against herbivory: comparison of wound- and insect elicitor-induced gene expression. New Phytologist, 172: 617–635. doi: 10.1111/j.1469-8137.2006.01877.x
- Issue published online: 19 SEP 2006
- Article first published online: 19 SEP 2006
- Received: 16 July 2006 Accepted: 26 July 2006
- defense gene expression;
- herbivore defense;
- hybrid poplar (Populus);
- plant defense;
- systemic response
- • In order to characterize defense responses of hybrid poplar (Populus trichocarpa× P. deltoides), we profiled leaf transcript patterns elicited by wounding and by regurgitant from forest tent caterpillar (FTC; Malacosoma disstria), a Lepidopteran defoliator of poplars.
- • Macroarrays were used to compare transcript profiles. Both FTC-regurgitant (FTC-R) and mechanical wounding with pliers elicited expression of a variety of genes, and for these genes our analysis indicated that these treatments induced qualitatively similar responses.
- • Similarily, a comparison of responses of directly treated and systemically induced leaves indicated extensive overlap in the sets of induced genes. FTC-R was found to contain the insect-derived elicitor volicitin.
- • The simulated herbivory treatments resulted in the induction of genes involved in poplar defense and secondary metabolism. We also identified wound-responsive genes with roles in primary metabolism, including a putative invertase, lipase, and acyl-activating enzyme; some of these genes may have roles in defense signaling. In addition, we found three unknown genes containing a ZIM motif which may represent novel transcription factors.