A transient decrease in reactive oxygen species in roots leads to root hair deformation in the legume–rhizobia symbiosis
Article first published online: 13 OCT 2006
Volume 173, Issue 1, pages 39–49, January 2007
How to Cite
Lohar, D. P., Haridas, S., Gantt, J. S. and VandenBosch, K. A. (2007), A transient decrease in reactive oxygen species in roots leads to root hair deformation in the legume–rhizobia symbiosis. New Phytologist, 173: 39–49. doi: 10.1111/j.1469-8137.2006.01901.x
- Issue published online: 13 OCT 2006
- Article first published online: 13 OCT 2006
- Received: 2 May 2005 Accepted: 24 August 2006
- Lotus japonicus;
- Medicago truncatula;
- oxidative burst;
- polar growth;
- reactive oxygen species;
- respiratory burst oxidase;
- root hair deformation;
- • A possible role for reactive oxygen species (ROS) in root hair deformation in response to Nod factor (NF) was investigated using Medicago truncatula nodulation mutants, and an inhibitor and precursors of ROS.
- • In wild-type roots, ROS efflux transiently decreased approximately 1 h after NF treatment. Transcript accumulation of two NADPH oxidase homologs, respiratory burst oxidase homolog 2 (MtRBOH2) and MtRBOH3, also transiently decreased at 1 h. However, in the nonnodulating mutant Nod factor perception (nfp), transcript accumulation did not change.
- • Exogenous application of ROS prevented root hair swelling and branching induced by NF. When accumulation of ROS was prevented by diphenylene iodonium (DPI), NF did not induce root hair branching. Root treatment with DPI alone reduced ROS efflux and induced root hair tip swelling. Transient treatment of roots with DPI mimicked NF treatment and resulted in root hair branching in the absence of NF. A transient DPI treatment did not induce root hair branching in the nonlegumes Arabidopsis thaliana and tomato (Lycopersicon esculentum).
- • The results suggest a role for the transient reduction of ROS accumulation in governing NF-induced root hair deformation in legumes.