Localization of water-soluble carbohydrates in wheat stems using imaging matrix-assisted laser desorption ionization mass spectrometry
Article first published online: 13 NOV 2006
Volume 173, Issue 2, pages 438–444, January 2007
How to Cite
Robinson, S., Warburton, K., Seymour, M., Clench, M. and Thomas-Oates, J. (2007), Localization of water-soluble carbohydrates in wheat stems using imaging matrix-assisted laser desorption ionization mass spectrometry. New Phytologist, 173: 438–444. doi: 10.1111/j.1469-8137.2006.01934.x
- Issue published online: 13 NOV 2006
- Article first published online: 13 NOV 2006
- Received: 11 August 2006 Accepted: 28 September 2006
- mass spectrometry;
- matrix-assisted laser desorption ionization (MALDI);
- wheat (Triticum aestivum)
- • The pool of endogenous water-soluble oligosaccharides found in the stems of wheat (Triticum aestivum) is being investigated as a potential indicator of grain yield. Techniques such as liquid chromatography with mass spectrometry (LC-MS) can profile these analytes but provide no spatial information regarding their distribution in the wheat stem. The imaging matrix-assisted laser desorption ionization (MALDI) mass spectrometry technique has not been utilized for the analysis of oligosaccharides in plant systems previously.
- • Imaging MALDI mass spectrometry was used to analyse cross and longitudinal sections from the stems of Triticum aestivum.
- • A range of oligosaccharides up to Hex11 were observed. Water-soluble oligosaccharides were ionized as potassiated molecules, and found to be located in the stem pith that is retained predominantly around the inner stem wall.
- • Imaging MALDI analyses provided spatial information on endogenous oligosaccharides present in wheat stems. The technique was found to offer comparable sensitivities for oligosaccharide detection to those of our established LC-MS method, and has potential for broad application in studying the in situ localization of other compound types in plant material.