In vivo visualization of F-actin structures during the development of the moss Physcomitrella patens
Article first published online: 15 JAN 2007
Volume 174, Issue 1, pages 63–76, April 2007
How to Cite
Finka, A., Schaefer, D. G., Saidi, Y., Goloubinoff, P. and Zrÿd, J.-P. (2007), In vivo visualization of F-actin structures during the development of the moss Physcomitrella patens. New Phytologist, 174: 63–76. doi: 10.1111/j.1469-8137.2007.01989.x
- Issue published online: 26 JAN 2007
- Article first published online: 15 JAN 2007
- Received: 2 October 2006 Accepted: 24 November 2006
- actin microfilament;
- cell polarity;
- green fluorescent protein (GFP)-talin;
- heat-shock promoter;
- Physcomitrella patens
- • The ‘in planta’ visualization of F-actin in all cells and in all developmental stages of a plant is a challenging problem. By using the soybean heat inducible Gmhsp17.3B promoter instead of a constitutive promoter, we have been able to label all cells in various developmental stages of the moss Physcomitrella patens, through a precise temperature tuning of the expression of green fluorescent protein (GFP)-talin.
- • A short moderate heat treatment was sufficient to induce proper labeling of the actin cytoskeleton and to allow the visualization of time-dependent organization of F-actin structures without impairment of cell viability.
- • In growing moss cells, dense converging arrays of F-actin structures were present at the growing tips of protonema cell, and at the localization of branching. Protonema and leaf cells contained a network of thick actin cables; during de-differentiation of leaf cells into new protonema filaments, the thick bundled actin network disappeared, and a new highly polarized F-actin network formed.
- • The controlled expression of GFP-talin through an inducible promoter improves significantly the ‘in planta’ imaging of actin.