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Fig. S1 Effects of GFPuv expression on in vitro bacterial growth

Fig. S2 Effect of GFPuv expression on in planta bacterial growth

Fig. S3 Correlation of fluorescence intensity and optical density at 600 nm during bacterial growth in broth

Fig. S4 Plants infected with pathogenic bacteria containing the plasmid pDSK-GFPuv

Table S1 Bacterial stains and plasmids used in this study

Movies S1 and S2 Intercellular movement of Pseudomonas syringae pv. tabaci in Nicotiana benthamiana leaves. Five-wk-old N. benthamiana plants were inoculated with P. syringae pv. tabaci containing the plasmid pDSK-GFPuv at the concentration of 2 x 104cfu ml-1 by vacuum infiltration and incubated at 20?22°C. Two days after inoculation, an infected leaf sample was mounted on a glass slide and the intercellular bacterial movement was monitored under a Leica confocal microscope with excitation at 488 nm and emitted light collected from 500 to 600 nm. Images for green fluorescence and light transmission were taken separately, under x20 magnification at 1 min interval for 2 h (Movie 1) and under x63 magnification at 5 s interval for 10 min (Movie 2), and merged together using Leica confocal software. Movies were made at a speed of 5 frames (images) per second

FilenameFormatSizeDescription
NPH_1999_sm_FigS1.pdf25KSupporting info item
NPH_1999_sm_FigS2.pdf22KSupporting info item
NPH_1999_sm_FigS3.pdf23KSupporting info item
NPH_1999_sm_FigS4.pdf211KSupporting info item
NPH_1999_sm_MovieS1.avi6667KSupporting info item
NPH_1999_sm_MovieS2.avi5937KSupporting info item
NPH_1999_sm_TableS1.pdf114KSupporting info item