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A quantitative trait loci analysis of Zinc hyperaccumulation in Arabidopsis halleri
Article first published online: 7 MAR 2007
DOI: 10.1111/j.1469-8137.2007.02036.x
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How to Cite
Filatov, V., Dowdle, J., Smirnoff, N., Ford-Lloyd, B., Newbury, H. J. and Macnair, M. R. (2007), A quantitative trait loci analysis of Zinc hyperaccumulation in Arabidopsis halleri. New Phytologist, 174: 580–590. doi: 10.1111/j.1469-8137.2007.02036.x
Publication History
- Issue published online: 7 MAR 2007
- Article first published online: 7 MAR 2007
- Received: 18 December 2006 Accepted: 17 January 2007
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Keywords:
- Arabidopsis lyrata;
- Arabidopsis petraea;
- Arabidopsis halleri;
- hyperaccumulation;
- quantitative trait locus (QTL);
- zinc (Zn)
Summary
- • The mechanisms of metal hyperaccumulation are still not understood, so we conducted a quantitative trait locus (QTL) analysis of zinc (Zn) hyperaccumulation in Arabidopsis halleri, in a cross between this and its sister species, A. petraea, in order to determine the number and approximate location of the genomic regions significantly contributing to this adaptation.
- • An F2 cross between the two species was made, and the leaf Zn concentration of 92 individuals was measured at both low (10 µm) and high (100 µm) Zn concentrations. Twenty-five markers were established that were distributed on all of the eight chromosomes.
- • Mapping of the markers established that they were essentially collinear with previous studies. QTLs exceeding a logarithm to the base 10 of the odds (LOD) value of 3 were found on chromosomes 4 (low Zn), 6 (high Zn) and 7 (both high and low Zn). Evidence for a QTL on chromosome 3 (low Zn) was also found.
- • This analysis validates a previously used method of QTL analysis, based on microarray analysis of segregating families. Genes that have altered during the evolution of this character should also be QTL: this analysis calls into question a number of candidate genes from consideration as such primary genes because they do not appear to be associated with QTLs.