Spatio-temporal leaf growth patterns of Arabidopsis thaliana and evidence for sugar control of the diel leaf growth cycle
Article first published online: 26 MAR 2007
© The Authors (2007). Journal compilation © New Phytologist (2007)
Volume 174, Issue 4, pages 752–761, June 2007
How to Cite
Wiese, A., Christ, M. M., Virnich, O., Schurr, U. and Walter, A. (2007), Spatio-temporal leaf growth patterns of Arabidopsis thaliana and evidence for sugar control of the diel leaf growth cycle. New Phytologist, 174: 752–761. doi: 10.1111/j.1469-8137.2007.02053.x
- Issue published online: 26 MAR 2007
- Article first published online: 26 MAR 2007
- Received: 13 December 2006 Accepted: 7 February 2007
- Arabidopsis thaliana;
- digital image sequence processing (DISP);
- leaf growth;
- sugar sensing
- • Leaf growth dynamics are driven by diel rhythms. The analysis of spatio-temporal leaf growth patterns in Arabidopsis thaliana wild type and mutants of interest is a promising approach to elucidate molecular mechanisms controlling growth. The diel availability of carbohydrates is thought to affect diel growth.
- • A digital image sequence processing (DISP)-based noninvasive technique for visualizing and quantifying highly resolved spatio-temporal leaf growth was adapted for the model plant A. thaliana. Diel growth patterns were analysed for the wild type and for a mutant with altered diel carbohydrate metabolism.
- • A. thaliana leaves showed highest relative growth rates (RGRs) at dawn and lowest RGRs at the beginning of the night. Along the lamina, a clear basipetal gradient of growth rate distribution was found, similar to that in many other dicotyledonous species. The starch-free 1 (stf1) mutant revealed changed temporal growth patterns with reduced nocturnal, and increased afternoon, growth activity.
- •The established DISP technique is presented as a valuable tool to detect altered temporal growth patterns in A. thaliana mutants. Endogenous changes in the diel carbohydrate availability of the starch-free mutant clearly affected its diel growth rhythms.