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Global monitoring of autumn gene expression within and among phenotypically divergent populations of Sitka spruce (Picea sitchensis)
Article first published online: 10 JAN 2008
DOI: 10.1111/j.1469-8137.2007.02346.x
© The Authors (2008).
Additional Information
How to Cite
Holliday, J. A., Ralph, S. G., White, R., Bohlmann, J. and Aitken, S. N. (2008), Global monitoring of autumn gene expression within and among phenotypically divergent populations of Sitka spruce (Picea sitchensis). New Phytologist, 178: 103–122. doi: 10.1111/j.1469-8137.2007.02346.x
Publication History
- Issue published online: 10 JAN 2008
- Article first published online: 10 JAN 2008
- Received: 18 November 2007Accepted: 21 November 2007
Keywords:
- adaptation;
- cold hardiness;
- genetic cline;
- microarray;
- real-time polymerase chain reaction (PCR);
- Sitka spruce (Picea sitchensis)
Summary
- •Cold acclimation in conifers is a complex process, the timing and extent of which reflects local adaptation and varies widely along latitudinal gradients for many temperate and boreal tree species. Despite their ecological and economic importance, little is known about the global changes in gene expression that accompany autumn cold acclimation in conifers.
- •Using three populations of Sitka spruce (Picea sitchensis) spanning the species range, and a Picea cDNA microarray with 21 840 unique elements, within- and among-population gene expression was monitored during the autumn. Microarray data were validated for selected genes using real-time PCR.
- •Similar numbers of genes were significantly twofold upregulated (1257) and downregulated (967) between late summer and early winter. Among those upregulated were dehydrins, pathogenesis-related/antifreeze genes, carbohydrate and lipid metabolism genes, and genes involved in signal transduction and transcriptional regulation. Among-population microarray hybridizations at early and late autumn time points revealed substantial variation in the autumn transcriptome, some of which may reflect local adaptation.
- •These results demonstrate the complexity of cold acclimation in conifers, highlight similarities and differences to cold tolerance in annual plants, and provide a solid foundation for functional and genetic studies of this important adaptive process.