These authors contributed equally to this paper.
Transcriptome analysis of Arabidopsis roots treated with signaling compounds: a focus on signal transduction, metabolic regulation and secretion
Article first published online: 18 APR 2008
© The Authors (2008). Journal compilation © New Phytologist (2008)
Volume 179, Issue 1, pages 209–223, July 2008
How to Cite
Badri, D. V., Loyola-Vargas, V. M., Du, J., Stermitz, F. R., Broeckling, C. D., Iglesias-Andreu, L. and Vivanco, J. M. (2008), Transcriptome analysis of Arabidopsis roots treated with signaling compounds: a focus on signal transduction, metabolic regulation and secretion. New Phytologist, 179: 209–223. doi: 10.1111/j.1469-8137.2008.02458.x
- Issue published online: 18 APR 2008
- Article first published online: 18 APR 2008
- Received: 28 December 2007; Accepted: 25 February 2008
Vol. 201, Issue 4, 1508, Article first published online: 6 JAN 2014
- defense response;
- methyl jasmonate (MeJA);
- nitric oxide (NO);
- root secretion;
- salicylic acid (SA);
- signaling molecules;
- • Gene expression in response to signaling molecules has been well studied in the leaves of the model plant species Arabidopsis thaliana. However, knowledge of gene expression and metabolic regulation at the root level is limited.
- • Here, the signaling compounds salicylic acid (SA), methyl jasmonate (MeJA) and nitric oxide (NO) were applied exogenously to induce various defense responses in roots, and their effect was studied using a combination of genomic, molecular and biochemical approaches. Genes involved in defense signaling/activation, cellular redox state, metabolism, transcription factors and membrane transport were altered in expression following treatment with SA, MeJA and NO.
- • In addition, it was found that SA-, MeJA- and NO-elicited roots increased the root exudation of phytochemicals compared with the roots of nontreated control plants. Transport systems likely to be involved in the root exudation of phytochemicals, including the MATE, ABC, MFS, amino acid, sugar and inorganic solute transporters, showed altered expression profiles in response to treatments.
- • Overall, significant differences were found in the signaling compound-elicited expression profiles of genes in roots vs those in leaves. These differences could be correlated to the underground nature of roots and their exposure to higher microbial inoculum rates under natural conditions.