Fig. S1 Comparison of inoculation methods. (a) Syringe, (b) vacuum, and (c) sap infiltration with vector B-PDS. Red arrows indicate photobleached leaves. (d) Percentage of plants showing photobleaching after sap inoculation with either the vector A-PDS (Ratcliffe et al. 2001) or B-PDS (Liu et al. 2002b) 10, 15, and 43 d post inoculation (dpi).

Fig. S2 Effect of temperature on silencing efficiency. (a) Percentage of PDS-silenced plants (vector B-PDS) grown at different temperatures showing photobleaching 16 and 22 d post inoculation (dpi). (b) Representative examples of Solanum nigrum treated with vectors B-PDS and grown at three different temperatures 22 dpi.

Table S1 Sequence information on primers used for cloning, vector generation, and quantitative real-time polymerase chain reaction (QRT-PCR).

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