Optimized virus-induced gene silencing in Solanum nigrum reveals the defensive function of leucine aminopeptidase against herbivores and the shortcomings of empty vector controls
Article first published online: 28 JUN 2008
© The Authors (2008). Journal compilation © New Phytologist (2008)
Volume 179, Issue 2, pages 356–365, July 2008
How to Cite
Hartl, M., Merker, H., Schmidt, D. D. and Baldwin, I. T. (2008), Optimized virus-induced gene silencing in Solanum nigrum reveals the defensive function of leucine aminopeptidase against herbivores and the shortcomings of empty vector controls. New Phytologist, 179: 356–365. doi: 10.1111/j.1469-8137.2008.02479.x
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- Received: 14 February 2008; Accepted: 17 March 2008
Fig. S1 Comparison of inoculation methods. (a) Syringe, (b) vacuum, and (c) sap infiltration with vector B-PDS. Red arrows indicate photobleached leaves. (d) Percentage of plants showing photobleaching after sap inoculation with either the vector A-PDS (Ratcliffe et al. 2001) or B-PDS (Liu et al. 2002b) 10, 15, and 43 d post inoculation (dpi).
Fig. S2 Effect of temperature on silencing efficiency. (a) Percentage of PDS-silenced plants (vector B-PDS) grown at different temperatures showing photobleaching 16 and 22 d post inoculation (dpi). (b) Representative examples of Solanum nigrum treated with vectors B-PDS and grown at three different temperatures 22 dpi.
Table S1 Sequence information on primers used for cloning, vector generation, and quantitative real-time polymerase chain reaction (QRT-PCR).
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