Spatial distribution of cadmium in leaves of metal hyperaccumulating Thlaspi praecox using micro-PIXE
Article first published online: 28 JUN 2008
© The Authors (2008). Journal compilation © New Phytologist (2008)
Volume 179, Issue 3, pages 712–721, August 2008
How to Cite
Vogel-Mikuš, K., Regvar, M., Mesjasz-Przybyłowicz, J., Przybyłowicz, W. J., Simčič, J., Pelicon, P. and Budnar, M. (2008), Spatial distribution of cadmium in leaves of metal hyperaccumulating Thlaspi praecox using micro-PIXE. New Phytologist, 179: 712–721. doi: 10.1111/j.1469-8137.2008.02519.x
- Issue published online: 15 JUL 2008
- Article first published online: 28 JUN 2008
- Received: 20 February 2008Accepted: 23 April 2008
- cadmium (Cd);
- dynamic analysis;
- metal hyperaccumulation;
- mineral nutrients;
- nuclear microprobe;
- Thlaspi praecox;
- true elemental mapping;
- X-ray micronalysis
- • Localization of cadmium (Cd) and other elements was studied in the leaves of the field-collected cadmium/zinc (Cd/Zn) hyperaccumulator Thlaspi praecox from an area polluted with heavy metals near a lead mine and smelter in Slovenia, using micro-PIXE (proton-induced X-ray emission).
- • The samples were prepared using cryofixation. Quantitative elemental maps and average concentrations in whole-leaf cross-sections and selected tissues were obtained.
- • Cd was preferentially localized in the lower epidermis (820 µg g−1 DW), vascular bundles and upper epidermis, whereas about twice the lower concentrations were found in the mesophyll.
- • Taking into account the large volume of the mesophyll compared with the epidermis, the mesophyll is indicated as a relatively large pool of Cd, possibly involved in Cd detoxification/dilution at the tissue and cellular level.