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Keywords:

  • Arabidopsis;
  • cell elongation;
  • PSK-alpha;
  • PSK receptor;
  • root growth

Summary

  • • 
    Phytosulfokine-α (PSK-α) is a disulfated pentapeptide described to act as a growth factor in suspension cells. In this study, the involvement of PSK signaling through the PSK receptor gene AtPSKR1 in Arabidopsis root growth was assessed.
  • • 
    Expression studies of PSK precursor genes and of AtPSKR1 were performed in roots with RT-PCR and P:GUS analyses. Root elongation, lateral root formation, cell production and root cell elongation were analyzed in wild-type (wt) and in the receptor knockout mutant Atpskr1-T treated with or without synthetic PSK-α.
  • • 
    Phytosulfokine and AtPSKR1 genes are differentially expressed in roots. PSK-α induced root growth in a dose-dependent manner without affecting lateral root density. Kinematic analysis established that enhancement of root growth by PSK-α was mainly caused by an increase in cell size. In Atpskr1-T, the primary roots were shorter as a result of reduced mature cell size and a smaller root apical meristem composed of fewer cells than in wt.
  • • 
    The results indicate that PSK-α signaling through AtPSKR1 affects root elongation primarily via control of mature cell size. Root organogenesis, on the other hand, is not controlled by PSK-α.