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Genome-wide reprogramming of regulatory networks, transport, cell wall and membrane biogenesis during arbuscular mycorrhizal symbiosis in Lotus japonicus
Article first published online: 15 JAN 2009
DOI: 10.1111/j.1469-8137.2008.02725.x
© The Authors (2009). Journal compilation © New Phytologist (2009)
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How to Cite
Guether, M., Balestrini, R., Hannah, M., He, J., Udvardi, M. K. and Bonfante, P. (2009), Genome-wide reprogramming of regulatory networks, transport, cell wall and membrane biogenesis during arbuscular mycorrhizal symbiosis in Lotus japonicus. New Phytologist, 182: 200–212. doi: 10.1111/j.1469-8137.2008.02725.x
Publication History
- Issue published online: 6 MAR 2009
- Article first published online: 15 JAN 2009
- Received: 5 September 2008, Accepted: 9 November 2008
Keywords:
- cell wall;
- Gigaspora margarita;
- laser microdissection;
- Lotus japonicus;
- mycorrhizal functioning;
- nutrient transporters;
- perifungal membrane
Summary
- • Arbuscular mycorrhizas (AMs) contribute significantly to soil nutrient uptake in plants. As a consequence of the fungal colonization and of the deep reorganization shown by arbusculated cells, important impacts on root transcriptome are expected.
- • An Affymetrix GeneChip with 50 000 probe-sets and real-time RT-PCR allowed us to detect transcriptional changes triggered in Lotus japonicus by the AM fungus Gigaspora margarita, when arbuscules are at their maximum (28 d postinoculation (dpi)). An early time (4 dpi) was selected to differentiate genes potentially involved in signaling and/or in colonization of outer tissues.
- • A large number (75 out of 558) of mycorrhiza-induced genes code for proteins involved in protein turnover, membrane dynamics and cell wall synthesis, while many others are involved in transport (47) or transcription (24). Induction of a subset (24 genes) of these was tested and confirmed by qRT-PCR, and transcript location in arbusculated cells was demonstrated for seven genes using laser-dissected cells.
- • When compared with previously published papers, the transcript profiles indicate the presence of a core set of responsive genes (25) that seem to be conserved irrespective of the symbiotic partner identity.