Fig. S1 Creation of knockout lines by replacement of Prx34A/34B genes in Physcomitrella patens with the hygromycin resistance gene.

Fig. S2 Kinetic curves of peroxidase activity in culture medium following chitosan treatment of Racomitrium japonicum.

Fig. S3 Phylogenetic tree based on comparison of the deduced amino acid sequences of 45 peroxidases from Physcomitrella patens and 138 peroxidases of rice (Oryza sativa).

Fig. S4 Peroxidase activity in chitosan-treated cultures from Prx34A/34B mutant moss lines.

Fig. S5 Growth of the hyphae of Irpex sp. on wild-type P. patens and a gene replacement mutant line.

Table S1 Positions of exons and introns in the predicted peroxidase genes of Physcomitrella patens.

Table S2 Primers used for quantitative PCR analysis of Prx34A/34B gene expression or gene replacement and analysis of the knockout mutants.

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