Fig. S1 Explant culture system for ethylene (ET) measurement.

Fig. S2 Phenotype and genotype of F1 and parents of the cross VFN × Never ripe.

Fig. S3 Phenotype of tomato leaflets infested with potato aphids in the choice assay (Fig. 3a) carried out with cv. Pearson (mi/mi nr/nr), Never ripe (mi/mi Nr/Nr), VFN (Mi-1/Mi-1 nr/nr), and VFN × Never ripe (Mi-1/Mi-1 Nr/Nr) plants.

Fig. S4 Effect of tobacco rattle virus (TRV) virus-induced gene silencing (VIGS) on transcript levels of 1-aminocyclopropane-1-carboxylic acid synthase 1A (ACS1A), ACS1B, ACS2 and ACS6 in control and silenced cv. Moneymaker (mi/mi) plants.

Table S1 Primers used for gene expression analyses

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