Symbiotic competence in Lotus japonicus is affected by plant nitrogen status: transcriptomic identification of genes affected by a new signalling pathway
Article first published online: 4 JUN 2009
© The Authors (2009). Journal compilation © New Phytologist (2009)
Volume 183, Issue 2, pages 380–394, July 2009
How to Cite
Omrane, S., Ferrarini, A., D’Apuzzo, E., Rogato, A., Delledonne, M. and Chiurazzi, M. (2009), Symbiotic competence in Lotus japonicus is affected by plant nitrogen status: transcriptomic identification of genes affected by a new signalling pathway. New Phytologist, 183: 380–394. doi: 10.1111/j.1469-8137.2009.02873.x
- Issue published online: 25 JUN 2009
- Article first published online: 4 JUN 2009
- Received: 19 February 2009, Accepted: 27 March 2009
- Lotus japonicus;
- nitrogen (N) supply;
- nodule organogenesis;
- nutritional status;
- signalling pathways;
- symbiotic competence
- • In leguminous plants, symbiotic nitrogen (N) fixation performances and N environmental conditions are linked because nodule initiation, development and functioning are greatly influenced by the amount of available N sources.
- • We demonstrate here that N supply also controls, beforehand, the competence of leguminous plants to perform the nodulation program. Lotus japonicus plants preincubated for 10 d in high-N conditions, and then transferred to low N before the Mesorhizobium loti inoculation, had reduced nodulation. This phenotype was maintained for at least 6 d and a complete reacquisition of the symbiotic competence was observed only after 9 d.
- • The time-course analysis of the change of the symbiotic phenotype was analysed by transcriptomics. The differentially expressed genes identified are mostly involved in metabolic pathways. However, the transcriptional response also includes genes belonging to other functional categories such as signalling, stress response and transcriptional regulation.
- • Some of these genes show a molecular identity and a regulation profile, that suggest a role as possible molecular links between the N-dependent plant response and the nodule organogenesis program.