Nitric oxide-induced rapid decrease of abscisic acid concentration is required in breaking seed dormancy in Arabidopsis
Article first published online: 12 JUN 2009
© The Authors (2009). Journal compilation © New Phytologist (2009)
Volume 183, Issue 4, pages 1030–1042, September 2009
How to Cite
Liu, Y., Shi, L., Ye, N., Liu, R., Jia, W. and Zhang, J. (2009), Nitric oxide-induced rapid decrease of abscisic acid concentration is required in breaking seed dormancy in Arabidopsis. New Phytologist, 183: 1030–1042. doi: 10.1111/j.1469-8137.2009.02899.x
- Issue published online: 10 AUG 2009
- Article first published online: 12 JUN 2009
- Received: 6 March 2009Accepted: 19 April 2009
- abscisic acid (ABA);
- nitric oxide (NO);
- (+)-abscisic acid 8′-hydroxylase
- • Nitric oxide (NO) has been reported to be involved in breaking seed dormancy but its mechanism of action is unclear.
- • Here, we report that a rapid accumulation of NO induced an equally rapid decrease of abscisic acid (ABA) that is required for this action in Arabidopsis.
- • Results of quantitative real-time polymerase chain reaction (QRT-PCR) and Western blotting indicate that the NO-induced ABA decrease correlates with the regulation of CYP707A2 transcription and (+)-abscisic acid 8′-hydroxylase (encoded by CYP707A2) protein expression. By analysing cyp707a1, cyp707a2 and cyp707a3 mutants, we found that CYP707A2 plays a major role in ABA catabolism during the first stage of imbibition.
- • Fluorescent images demonstrate that NO is released rapidly in the early hours at the endosperm layer during imbibition. Evidently, such response precedes the enhancement of ABA catabolism which is required for subsequent seed germination.