Exploding a myth: the capsule dehiscence mechanism and the function of pseudostomata in Sphagnum
Article first published online: 22 JUN 2009
© The Authors (2009). Journal compilation © New Phytologist (2009)
Volume 183, Issue 4, pages 1053–1063, September 2009
How to Cite
Duckett, J. G., Pressel, S., P’ng, K. M. Y. and Renzaglia, K. S. (2009), Exploding a myth: the capsule dehiscence mechanism and the function of pseudostomata in Sphagnum. New Phytologist, 183: 1053–1063. doi: 10.1111/j.1469-8137.2009.02905.x
- Issue published online: 10 AUG 2009
- Article first published online: 22 JUN 2009
- Received: 26 February 2009Accepted: 19 April 2009
- cryoscanning electron microscopy;
- spore discharge;
- • The nineteenth century air-gun explanation for explosive spore discharge in Sphagnum has never been tested experimentally. Similarly, the function of the numerous stomata ubiquitous in the capsule walls has never been investigated.
- • Both intact and pricked Sphagnum capsules, that were allowed to dry out, all dehisced over an 8–12 h period during which time the stomatal guard cells gradually collapsed and their potassium content, measured by X-ray microanalysis in a cryoscanning electron microscope, gradually increased. By contrast, guard cell potassium fell in water-stressed Arabidopsis.
- • The pricking experiments demonstrate that the air-gun notion for explosive spore discharge in Sphagnum is inaccurate; differential shrinkage of the capsule walls causes popping off the rigid operculum. The absence of evidence for a potassium-regulating mechanism in the stomatal guard cells and their gradual collapse before spore discharge indicates that their sole role is facilitation of sporophyte desiccation that ultimately leads to capsule dehiscence.
- • Our novel functional data on Sphagnum, when considered in relation to bryophyte phylogeny, suggest the possibility that stomata first appeared in land plants as structures that facilitated sporophyte drying out before spore discharge and only subsequently acquired their role in the regulation of gaseous exchange.