Ethylene is involved in nitrate-dependent root growth and branching in Arabidopsis thaliana
Article first published online: 1 SEP 2009
© The Authors (2009). Journal compilation © New Phytologist (2009)
Special Issue: Featured papers on ‘Weeds - bridging the gap between evolutionary ecology and crop science’
Volume 184, Issue 4, pages 918–931, December 2009
How to Cite
Tian, Q.-Y., Sun, P. and Zhang, W.-H. (2009), Ethylene is involved in nitrate-dependent root growth and branching in Arabidopsis thaliana. New Phytologist, 184: 918–931. doi: 10.1111/j.1469-8137.2009.03004.x
- Issue published online: 6 NOV 2009
- Article first published online: 1 SEP 2009
- Received: 6 July 2009, Accepted: 13 July 2009
- Arabidopsis thaliana;
- ethylene production;
- lateral root development;
- nitrate concentration;
- nitrate transporters
- •Here, we investigated the role of ethylene in high nitrate-induced change in root development in Arabidopsis thaliana using wild types and mutants defective in ethylene signaling (etr1, ein2) and nitrate transporters (chl1, nrt2.1).
- •The length and number of visible lateral roots (LRs) were reduced upon exposure of wild-type seedlings grown on low (0.1 mm) to high nitrate concentration (10 mm). There was a rapid burst of ethylene production upon exposure to high nitrate concentration.
- •Ethylene synthesis antagonists, cobalt (Co2+) and aminoethoxyvinylglycine (AVG), mitigated the inhibitory effect of high nitrate concentration on lateral root growth. The etr1-3 and ein2-1 mutants exhibited less reductions in LR length and number than wild-type plants in response to high nitrate concentration. Expression of nitrate transporters AtNRT1.1 and AtNRT2.1 was upregulated and downregulated in response to high nitrate concentration, respectively. A similar upregulation and downregulation of AtNRT1.1 and AtNRT2.1 was observed by ethylene synthesis precursor aminocyclopropane carboxylic acid (ACC) and AVG in low and high nitrate concentration, respectively. Expression of AtNRT1.1 and AtNRT2.1 became insensitive to high nitrate concentration in etr1-3 and ein2-1 plants.
- •These findings highlight the regulatory role that ethylene plays in high nitrate concentration-regulated LR development by modulating nitrate transporters.