These authors contributed equally to this work as first authors.
Poplar defense against insects: genome analysis, full-length cDNA cloning, and transcriptome and protein analysis of the poplar Kunitz-type protease inhibitor family
Version of Record online: 23 SEP 2009
© The Authors (2009). Journal compilation © New Phytologist (2009)
Special Issue: Featured papers on ‘Weeds - bridging the gap between evolutionary ecology and crop science’
Volume 184, Issue 4, pages 865–884, December 2009
How to Cite
Philippe, R. N., Ralph, S. G., Külheim, C., Jancsik, S. I. and Bohlmann, J. (2009), Poplar defense against insects: genome analysis, full-length cDNA cloning, and transcriptome and protein analysis of the poplar Kunitz-type protease inhibitor family. New Phytologist, 184: 865–884. doi: 10.1111/j.1469-8137.2009.03028.x
- Issue online: 6 NOV 2009
- Version of Record online: 23 SEP 2009
- Received: 1 June 2009, Accepted: 28 July 2009
Methods Microarray analysis, qRT-PCR analysis, analysis of poplar Kunitz protease inhibitor (pKPI) proteins in insect gut.
Fig. S1 Amino acid sequence alignment of poplar Kunitz protease inhibitor (KPI) proteins.
Fig. S2 Schematic of stress treatment conditions.
Fig. S3 Schematic of the microarray hybridizationdesigns.
Fig. S4 qRT-PCR analysis of gene expression inindividual trees in response to mechanical wounding plus forest tent caterpillar oral secretions.
Table S1 Poplar genotypes used in this study
Table S2 Oligonucleotide primers used in qRT-PCRanalyses
Table S3 Poplar Kunitz protease inhibitor (pKPI)amino acid sequence identity matrix
Table S4 Allelic groups from various poplar genotypes
Table S5 Comparison of qRT-PCR analyses using pooledvs individual replicates
Table S6 qRT-PCR analysis of transcript abundance for 11 poplar Kunitz protease inhibitor (KPI) genes in response to FTC feeding, MW, OS and MeJa treatment
Table S7 Gene models found adjacent to a Kunitz protease inhibitor (KPI) onpoplar linkage groups (chromosomes), as shown in Fig. 1
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