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Variability of zinc tolerance among and within populations of the pseudometallophyte species Arabidopsis halleri and possible role of directional selection

Authors

  • Claire-Lise Meyer,

    1. Laboratoire de Génétique et Évolution des Populations Végétales, UMR CNRS 8016, Université des Sciences et Technologies de Lille, Bâtiment SN2, F–59655 Villeneuve d’Ascq Cedex, France
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    • These authors contributed equally to this work.

  • Alicja A. Kostecka,

    1. Department of Ecology, Institute of Botany Polish Academy of Sciences, Lubicz 46, PL–31 512 Cracow, Poland
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    • These authors contributed equally to this work.

  • Pierre Saumitou-Laprade,

    1. Laboratoire de Génétique et Évolution des Populations Végétales, UMR CNRS 8016, Université des Sciences et Technologies de Lille, Bâtiment SN2, F–59655 Villeneuve d’Ascq Cedex, France
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  • Anne Créach,

    1. Laboratoire de Génétique et Évolution des Populations Végétales, UMR CNRS 8016, Université des Sciences et Technologies de Lille, Bâtiment SN2, F–59655 Villeneuve d’Ascq Cedex, France
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  • Vincent Castric,

    1. Laboratoire de Génétique et Évolution des Populations Végétales, UMR CNRS 8016, Université des Sciences et Technologies de Lille, Bâtiment SN2, F–59655 Villeneuve d’Ascq Cedex, France
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  • Maxime Pauwels,

    1. Laboratoire de Génétique et Évolution des Populations Végétales, UMR CNRS 8016, Université des Sciences et Technologies de Lille, Bâtiment SN2, F–59655 Villeneuve d’Ascq Cedex, France
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  • Hélène Frérot

    1. Laboratoire de Génétique et Évolution des Populations Végétales, UMR CNRS 8016, Université des Sciences et Technologies de Lille, Bâtiment SN2, F–59655 Villeneuve d’Ascq Cedex, France
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Author for correspondence:
Claire-Lise Meyer
Tel: +33 320434033
Email: claire-lise.meyer@ed.univ-lille1.fr

Summary

  •  We estimated the level of quantitative polymorphism for zinc (Zn) tolerance in neighboring metallicolous and nonmetallicolous populations of Arabidopsis halleri and tested the hypothesis that divergent selection has shaped this polymorphism.
  •  A short-term hydroponic test was used to capture the quantitative polymorphism present between edaphic types, among and within populations. We measured six morphological and physiological traits on shoots and roots to estimate the response of A. halleri to Zn. In order to assess the adaptive value of Zn tolerance polymorphism, we compared differentiation of quantitative traits with that of molecular markers.
  •  Zinc tolerance of metallicolous populations was, on average, higher than that of nonmetallicolous populations according to the morphological and physiological traits measured. Phenotypic variability within edaphic types was very high and mainly explained by polymorphism among individuals within populations. Genetic differentiation for photosystem II yield of leaves (a measure of photosynthetic efficiency) was greater than the differentiation for microsatellite and thus, probably shaped by divergent selection.
  •  Overall, these results suggest that, in the sampled populations, Zn tolerance has been increased in metallicolous populations through selection on standing genetic variation within local nonmetallicolous ancestral populations.

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