Membrane steroid-binding protein 1 induced by a diffusible fungal signal is critical for mycorrhization in Medicago truncatula
Article first published online: 14 DEC 2009
© The Authors (2009). Journal compilation © New Phytologist (2009)
Volume 185, Issue 3, pages 716–733, February 2010
How to Cite
Kuhn, H., Küster, H. and Requena, N. (2010), Membrane steroid-binding protein 1 induced by a diffusible fungal signal is critical for mycorrhization in Medicago truncatula. New Phytologist, 185: 716–733. doi: 10.1111/j.1469-8137.2009.03116.x
- Issue published online: 15 JAN 2010
- Article first published online: 14 DEC 2009
- Received: 10 September 2009, Accepted: 6 October 2009
- arbuscular mycorrhiza symbiosis;
- diffusible fungal signal;
- Glomus intraradices;
- Medicago truncatula (barrel medic);
- MtMSBP1 membrane steroid-binding protein;
- SYM Pathway
- •Arbuscular mycorrhiza (AM) is a mutualistic biotrophic association that requires a complex exchange of signals between plant and fungus to allow accommodation of the mycosymbiont in the root cortex. Signal exchange happens even before physical contact, activating the plant symbiotic program.
- •We investigated very early transcriptional responses in Medicago truncatula to inoculation with Glomus intraradices and identified four genes induced by diffusible AM fungal signals before contact. Three of them were previously shown to be mycorrhiza induced at later stages of the symbiosis, while MtMSBP1, encoding a membrane-bound steroid-binding protein, is a novel mycorrhizal marker.
- •Expression analyses in plants defective in the symbiotic receptor kinase DMI2 allowed discrimination of two different signaling cascades involved in the perception of the diffusible signals. Thus, while some of the genes are activated in a DMI2-dependent manner, the induction of one of them encoding a proteinase inhibitor is DMI2-independent.
- •Downregulation of MtMSBP1 by RNAi led to an aberrant mycorrhizal phenotype with thick and septated appressoria, decrease number of arbuscules and distorted arbuscule morphology. This provides genetic evidence that MtMSBP1 is critical for mycorrhiza development. We hypothesize that MtMSBP1 plays a role in sterol homeostasis in the root.